Efficient gene transfection using chitosan ? alginate core-shell nanoparticles

Efficient gene transfection using chitosan–alginate core-shell nanoparticles

Reverse microemulsion was used as a template to fabricate chitosan-alginate core-shell nanoparticles encapsulated with enhanced green fluorescent protein (EGFP)-encoded plasmids. The average size of DNA-entrapped nanoparticles measured by dynamic light scattering was increased proportionally, with the N/P ratios ranging from 5 to 20. These alginate-coated chitosan nanoparticles endocytosed by N...

Photochemical internalization-mediated nonviral gene transfection: polyamine core-shell nanoparticles as gene carrier.

The overall objective of the research was to investigate the utility of photochemical internalization (PCI) for the enhanced nonviral transfection of genes into glioma cells. The PCI-mediated introduction of the tumor suppressor gene phosphatase and tensin homolog (PTEN) or the cytosine deaminase (CD) pro-drug activating gene into U87 or U251 glioma cell monolayers and multicell tumor spheroids...

Magnetic core-shell chitosan nanoparticles: rheological characterization and hyperthermia application.

Stabilized magnetic nanoparticles are the subject of intense research for targeting applications and this work deals with the design, preparation and application of specific core-shell nanoparticles based on ionic crosslinked chitosan. The nanometric size of the materials was demonstrated by dynamic light scattering (DLS) and field emission scanning electron microscopy (FESEM) that also proved ...

A novel method of cinnamon oil nanocapsulation in core-shell chitosan-alginate by freeze dryer

The objective of this work was to characterize the cinnamon oil nanocapsule that contained alginate-chitosan as coating agents. In this work, cinamonn oil loaded chitosan nanoparticles (CS-alginate NP-cinamonn) are prepared by a two-step process including oil/water emulsion and ionic gelation. In this study, cinamon as a core material was nanoencapsulated with chitosan alginate at a ratio of 1:...

Chitosan-graft-PAMAM / alginate core-shell nanoparticles: A safe and promising oral insulin carrier in Animal Model